BIOTECHNOLOGY

BIOTECHNOLOGY

Consider the DNA sequence below:

3’ – T A C C A A G G A G C A T T A G A T A C T – 5’

5’ – A T G G T T C C T C G T A A T C T A T G A – 3’

  1. Write the mRNA sequence that would be produced if the TOP DNA strand were used as the template strand (label the 5’ and 3’ ends):
  2. What is the amino acid sequence of the protein that would be made using the mRNA above in part a ?
  3. Write the mRNA sequence that would be produced if the BOTTOM DNA strand were used as the template strand :
  4. What is the amino acid sequence of the protein that would be made using the mRNA above in part c ?
  5. What is the amino acid sequence of the protein that would be made using the mRNA above in part c ?

mRNA is always from 5′->3′ and same as the sense strand or template strand except change T of DNA into U for RNA

DNA sequence:

3’ – T A C C A A G G A G C A T T A G A T A C T – 5’

  1. A) mRNA sequence:

5′ – U C A U A G A U U A C G A G G A A C C A U – 3′

  1. B) Amino acid sequence:

Ser – stop

As ,UAG is a stop codon

DNA sequence:

5’ – A T G G T T C C T C G T A A T C T A T G A – 3’

  1. C) mRNA sequence:

5′- A U G G U U C C U C G U A A U C U A U G A – 3′

  1. D) Amino acid sequence :

Met Val Pro ArgAsnLeu stop

UGA is a stop codon

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