CLONING IN PLANTS and DNA CLONING

CLONING IN PLANTS :

1) Cuttings

The best manner to clone a plant is taking a cuttings. A branch from the parent plant is cut off, its lower leaves are eliminated, and the stem is planted in damp compost. Plant hormones are regularly used for new roots to expand. After some weeks, new roots grow and a new plant is produced. The method is mostly used by gardeners.

2) Tissue culture :

Another manner of cloning plants is by means of tissue culture. Sterile agar jelly with plant hormones and masses of nutrients is required. Tissue culture is more expensive and tough to do than taking cuttings.

DNA CLONING :

DNA cloning is a molecular biology method that makes many equal copies of a bit of DNA, consisting of a gene.

In a cloning experiment, a targetted gene is inserted into a circular piece of DNA called a plasmid.

The plasmid is introduced into micro organism via manner known as transformation, and micro organism sporting the plasmid are selected on using antibiotics.

Bacteria with the precise plasmid are used to make plasmid DNA or, in some instances, caused to express the gene and make protein…..

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