How do you clone a piece of DNA?
Asked By Shailja
Replied by Admin
Cloning is an asexual biological process in which identical copies or exact replica of anything can be produced. How to clone a piece of DNA DNA cloning is a molecular biology technique that makes many identical copies of a piece of DNA, such as a gene. In a typical cloning experiment, a target gene is inserted into a circular piece of DNA called a plasmid. The plasmid is introduced into bacteria via process called transformation, and bacteria carrying the plasmid are selected using antibiotics. Bacteria with the correct plasmid are used to make more plasmid DNA or, in some cases, induced to express the gene and make protein. Steps in DNA cloning are: 1) To clone a piece of DNA, firstly the chosen piece of DNA is ‘cut’ from the source organism using restriction enzymes. 2) The piece of DNA is then ‘pasted’ into a vector and the ends of the DNA are joined with the vector DNA by ligation. DNA that has been ‘cut’ and ‘pasted’ from an organism into a vector is called recombinant DNA. Therefore, DNA cloning also called as recombinant DNA technology. 3) The vector is introduced into a host cell such as bacterium or yeast by a process called transformation. The host cells copy the vector DNA along with their own DNA that make multiple copies of the inserted DNA. 4) The vector DNA is isolated from the host cells DNA and then purified....
- 11 Nov 2017 @ 06:07